Surface engineering can be a powerful technique for dealing with proteins that yield no crystals or poorly diffracting crystals. The JBScreen Methylation Kit provides a simple method for the selective methylation of lysine residues. The method does not require laborious cloning/expression/purification. Instead, the protons of the amino groups of all lysines are chemically replaced with methyl groups. A surface-engineered protein, ready for crystallization, is produced within 24 hours.Each JBScreen Methylation Kit contains all necessary reagents for six methylation experiments. All components are provided ready to use, so just follow the manual step-by-step. No background in chemistry necessary.
Download User Manual below:JBS Methylation Kit User Guide
JBScreen pH-2D allows sampling of a broad pH range without changing the buffering environment [1]. This ensures that pH screening is independent of the chemical nature of the components of the buffer system.
JBScreen pH-2D allows sampling of a broad pH range without changing the buffering environment [1]. This ensures that pH screening is independent of the chemical nature of the components of the buffer system.JBScreen pH-2D contains six extended range buffer systems, each composed of a mixture of three individual buffers with a distinct chemical nature and well separated pKa values. Each buffer system is composed of a low-pH and a high-pH stock solution, supplied in 10 ml volumes. The low-pH stock solution is preset at pH 4.0 and the high-pH solution at either pH 9.0 or 10.0 (see download). Thus, mixtures of the low-pH and high-pH stock solutions in different proportions provide coverage of the entire pH range from 4.0 – 9.0 or 4.0 – 10.0, respectively. The composition of the buffer systems assures a virtually linear distribution of the resulting pH vs the percentage of high pH / low pH stock.
Larger volumes are available upon request.
[1] Newman (2004) Novel buffer systems for macromolecular crystallization. Acta Cryst. D60:610.
Note: This is a special order item with 2-4 weeks delivery , during our initial phase of carrying Jena products. If you would like us to stock this item, please let us know!
Download technical sheet from below:JBScreen pH-2D
The Universal V1-Puck (Unipuck) is a sample pin storage and shipping container compatible with many automated sample mounting systems. The V1-puck holds 16 MiTeGen sample bases/pins. It consists of two parts shown in the image: 1) the sample enclosure (left) and 2) the sample base piece (right).
Each part of the V1-puck has a unique serial number for identification. Custom serial numbers are available if requested at time of order.
For more information on the development of the V1-puck visit: http://smb.slac.stanford.edu/robosync.
JBScreen Plus is an additive screen for optimization of preliminary crystallization conditions. The selection of the additives is based upon the Hofmeister series, which orders ions based upon their ability to stabilize protein structure.
JBScreen Plus
JBScreen Plus 是一种用于优化初步结晶条件的添加剂筛。 添加剂的选择基于 Hofmeister 系列，它根据离子稳定蛋白质结构的能力对它们进行排序。
Full Description
Category: Crystallization Optimization
Product Information
Product Description
Product Specification
JBScreen Plus is an additive screen for optimization of preliminary crystallization conditions. The selection of the additives is based upon the Hofmeister series, which orders ions based upon their ability to stabilize protein structure. Ions can be classified as either kosmotropic or chaotropic. The first have structure stabilizing properties, and may assist in, e.g. crystallizing proteins with a large proportion of flexible loop regions. The latter have structure disturbing properties, and may assist in the crystallization of large complexes, allowing them to re-arrange to form favorable crystal contacts.
JBScreen Plus consists of 5 individual kits, JBScreen Plus Kosmotropic, JBScreen PlusChaotropic, JBScreen Plus Salts, JBScreen Plus Additives and JBScreen Plus Volatiles, containing 24 different additives each. The ready-to-use reagents are supplied in 1 ml aliquots.
The 96 solutions of JBScreen Plus HTS, comprising the reagents of the kosmotropic, chaotropic, salts and additive kits, are supplied in a sterile deep well block containing either 200 µl (JBScreen Plus HTS S) or 1 ml (JBScreen Plus HTS L).
JBScreen Plus HTS S is particularly designed for low volume users. The screening reagents can be added directly to the additives in the deep well block before starting a vapor diffusion experiment, while JBScreen Plus HTS L will enable you to transfer any desired volume of additive solution to your plate.
If you prefer not to order online, click here for a printable fill-in order form.
Note: These are special order items with 2-4 weeks delivery , during our initial phase of carrying Jena products. If you would like us to stock these items, please let us know!
Download technical sheet from below:JBScreen Plus Volatiles, 1 kit JBScreen Plus HTS L (1.0 per well), 1 block JBScreen Plus Kosmotropic, 1 kit JBScreen Plus Chaotropic, 1 kit JBScreen Plus Salts, 1 kit JBScreen Plus Additives, 1 kit
类别：结晶优化
产品信息
产品描述
产品规格
JBScreen Plus 是一种用于优化初步结晶条件的添加剂筛。添加剂的选择基于 Hofmeister 系列，它根据离子稳定蛋白质结构的能力对它们进行排序。离子可分为亲液性或离液性。第一个具有结构稳定特性，并且可能有助于，例如结晶具有大部分柔性环区域的蛋白质。后者具有结构干扰特性，可能有助于大配合物的结晶，使它们重新排列以形成有利的晶体接触。
JBScreen Plus 由 5 个独立的套件组成，JBScreen Plus Kosmotropic、JBScreen PlusChaotropic、JBScreen Plus Salts、JBScreen Plus Additives 和 JBScreen Plus Volatiles，每个套件包含 24 种不同的添加剂。即用型试剂以 1 ml 等分试样提供。
JBScreen Plus HTS 的 96 种溶液，包括亲液、离液、盐和添加剂试剂盒的试剂，以无菌深孔块形式提供，含有 200 µl (JBScreen Plus HTS S) 或 1 ml (JBScreen Plus HTS L)。
JBScreen Plus HTS S 专为低音量用户设计。在开始蒸汽扩散实验之前，筛选试剂可以直接添加到深孔模块中的添加剂中，而 JBScreen Plus HTS L 将使您能够将任何所需体积的添加剂溶液转移到您的板上。
从下面下载技术表：
JBScreen Plus Volatiles，1 套件 JBScreen Plus HTS L（每孔 1.0），1 块 JBScreen Plus Kosmotropic，1 套件 JBScreen Plus Chaotropic，1 套件 JBScreen Plus 盐，1 套件 JBScreen Plus 添加剂，1 套件
Add a barcode to your Unipuck, for use at facilities such as Diamond Light Source or Swiss Light Source. Barcodes allow facilities to keep easier track of samples during automated data collection.
Single stock solutions of the JBScreen components, i.e. buffers, salts and precipitants, are ideal for the optimization of your crystallization conditions.
Using the same chemicals as utilized in the JBScreens ensures higher reproducibility of your experiments. JBScreen Single Stocks are ready for use: the concentration is adjusted and they are sterile filtered.
JBScreen 组分的单一储备溶液，即缓冲液、盐和沉淀剂，是优化结晶条件的理想选择。
使用与 JBScreens 相同的化学品可确保您的实验具有更高的重现性。 JBScreen Single Stocks 即可使用：调整浓度并无菌过滤。
Single stock solutions of the JBScreen components, i.e. buffers, salts and precipitants, are ideal for the optimization of your crystallization conditions.
Using the same chemicals as utilized in the JBScreens ensures higher reproducibility of your experiments. JBScreen Single Stocks are ready for use: the concentration is adjusted and they are sterile filtered.
JBScreen 组分的单一储备溶液，即缓冲液、盐和沉淀剂，是优化结晶条件的理想选择。
使用与 JBScreens 相同的化学品可确保您的实验具有更高的重现性。 JBScreen Single Stocks 即可使用：调整浓度并无菌过滤。
UniPucks come standard with engraved serialization from the factory (e.g. CPS-0583).
Alternatively if specified at the time of purchase you have the option to have the pucks custom engraved at no extra charge (order part number M-CP-CENGR). The format for the custom serial engraving is limited to three (3) CAPITAL letters then a dash followed by three (3) numbers. Here is an example, ABC-001 to ABC-007.
JBScreen Solubility HTS, developed by Meindert Lamers from the MRC in Cambridge, is designed to quickly find suitable buffer components to purify and store protein in.
JBScreen Solubility HTS 由来自剑桥大学 MRC 的 Meindert Lamers 开发，旨在快速找到合适的缓冲液成分来纯化和储存蛋白质。
JBScreen Solubility HTS tests for buffer, pH, salt and glycerol at the same time: For all proteins investigated, suitable conditions were found in a single assay. Protein and buffer conditions are dispensed in a ratio of 1:3, thereby minimizing the effect of any buffer components in which the protein is initially stored. Standard crystallization robots set up the assay in 5 minutes and very small amounts of protein are required (10 µl @ 5-10 mg/ml). The results are visible after one hour at high protein concentration or 12 hours at low protein concentration:
Get your Uni-pucks set in a series of unique colors. Help identify which puck you are working with. Available in 7 different colors, or one unique color.
Individual colors:
Black
Red
Orange (Not shown)
Green
Blue
Purple
Grey
(This is an add-on option, Catalog number M-CP-CustomColors and should be selected in addition to and at the same time as a set of pucks. Minimum of 7 pucks required.)
Examples of a drop without protein precipitation (left) and a drop with protein precipitation (right)
