SPINE Pucks (SC3 Baskets) and Tools-MiTeGen蛋白结晶

上海金畔生物代理MiTeGen品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问MiTeGen官网或者咨询我们获取更多相关MiTeGen品牌产品信息。

SPINE Pucks (SC3 Baskets) and Tools

SPINE Pucks (SC3 Baskets) and Tools

SPINE Pucks (SC3 Baskets) and Tools SKU: M-SPSK-001 $2,575.00
SPINE Pucks (SC3 Baskets) and Tools SKU: M-SP-SC3-1 $345.00
SPINE Pucks (SC3 Baskets) and Tools SKU: M-SP-PTT-1 $375.00
SPINE Pucks (SC3 Baskets) and Tools SKU: M-SP-SPH-1 $375.00
SPINE Pucks (SC3 Baskets) and Tools SKU: M-SP-ECT $20.00

Categories: Crystallography Pucks & Cassettes, Pucks, SPINE (SC3) Baskets

Product Information

  • Puck for SPINE Format Sample Holders
  • SPINE Puck Transfer Tool
  • Shelved Puck Holder

These pucks can be loaded with 10 frozen samples mounted on SPINE format holders. Each puck is identified by an unique dot matrix. The SPINE standard is the sample holder format accepted by most of the sample changers available on MX beamlines in Europe, and elsewhere.

SPINE Pucks (SC3 Baskets) and Tools

M-CSM003-0001A SPINE PUCKS (SC3 BASKETS)

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    CryoVials help keep your sample cold during transfer from a dewar to a cold gas stream. They also provide…

Reusable Crystal Mount, Base Assemblies

SPINE Pucks (SC3 Baskets) and Tools
MiTeGen offers ready-to-use assemblies for cryocrystallography. The mounts/loops can be epoxied into standard…

CombiPuck System

SPINE Pucks (SC3 Baskets) and Tools
The CombiPuck™ system enables crystallographers to send crystals stored in SPINE standard magnetic…

Magnetic CryoVials

SPINE Pucks (SC3 Baskets) and Tools
CryoVials help keep your sample cold during transfer from a dewar to a cold gas stream. They also provide…

LV CryoOil-MiTeGen蛋白结晶

上海金畔生物代理MiTeGen品牌蛋白结晶试剂耗材工具等,我们将竭诚为您服务,欢迎访问MiTeGen官网或者咨询我们获取更多相关MiTeGen品牌产品信息。

LV CryoOil

LV CryoOil

  • Superior Cryoprotectant
  • Ultra-low viscosity
  • Minimal background scatter
LV CryoOil SKU: LVCO-1 $47.00
LV CryoOil SKU: LVCO-5 $62.00
LV CryoOil SKU: LVCO-50 $238.00

Categories: Crystal Harvesting, Oils & Grease, Cryo-EM Grid Boxes and Accessories, Accessories

Product Information

  • Product Description and Usage
  • Why Mitegen’s LV CryoOil™

Oils are widely used in crystallography – as cryoprotectants when samples are flash cooled, and as barriers to the dehydration, solvent evaporation, and oxidation that may occur in air near and above room temperature.

The ice rings seen in the diffraction patterns of flash cooled protein crystals arise primarily from crystallization of the aqueous solution surrounding the crystal, not of the internal solvent. Oils can displace and replace this surrounding solution with little risk of damage due to osmotic shock.
Advances in X-ray sources, optics, detectors and sample mounting technology have allowed protein crystals as small as 5 microns to be used in structure determinations. Crystals smaller than roughly 50 microns dehydrate rapidly in air, with sub-10 micron crystals dehydrating in seconds.

Substantial dehydration-induced damage can easily occur between removal from a drop and flash cooling. Dehydration can be minimized by first transferring to an oil drop (using the MicroSieves™ in Microtools™ Kit 1 and Microtools™ Kit 2 ) before mounting the oil-coated crystal on a MicroMount or MicroMesh.

However, surrounding oil scatters X-rays, reducing diffraction signal-to-noise, and contributes thermal mass that reduces crystal cooling rates and increases the need for penetrating cryoprotectants. These problems are especially acute for the smallest crystals. When mineral oil, Paratone oil and other high viscosity oils are used, the volume of surrounding oil can exceed the crystal volume by a factor of 10 or more, and can contribute excess drag that increases sample motion in a cryostream. For larger rod or plate shaped crystals, the surrounding oil exerts stresses when it freezes that can cause crystal cracking and mosaic broadening.

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