Chemically competent E. coli cells suitable for high efficiency transformation and isolation of plasmid clones containing repeat elements.

Highlights

Transformation efficiency:
1-3 x 109 cfu/μg pUC19 DNA

Recommended host strain for cloning genes into retroviral/ lentiviral vectors

Recommended for cloning of direct repeats and inverted repeat sequences

Reduced recombination of cloned DNA (recA1)

Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]

Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations

Resistance to phage T1 (fhuA)

Suitable for blue/white screening with vectors capable of alpha-complementation

Genotype

F’ proA+B+ lacIq ?(lacZ)M15 zzf::Tn10 (TetR)/ ?(ara-leu) 7697 araD139 fhuA ?lacX74 galK16 galE15 e14- ?Φ80dlacZ?M15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 ?(mrr-hsdRMS-mcrBC)

Reagents Supplied

The following reagents are supplied with this product:

NEB #

Component Name

Component #

Stored at (°C)

Amount

Concentration

C3040H

-80

NEB? 10-beta/Stable Outgrowth Medium

B9035SVIAL

4

1 x 25 ml

Not Applicable

pUC19 Vector

N3041AVIAL

-20

1 x 0.025 ml

50 pg/μl

NEB? Stable Competent E. coli (High Efficiency)

C3040HVIAL

-80

20 x 0.05 ml

Not Applicable

D、销售的所有产品均不得用于人类或动物之临床诊断或治疗，仅可用于工业或者科研等非医疗目的。