描述
M-CSF Protein, Mouse is a pro-inflammatory cytokine, binds to its receptor CSF1R, and is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes.

研究背景
Macrophage Colony Stimulating Factor (M-CSF) is a pro-inflammatory cytokine, constitutively produced by several cell types, such as fibroblasts, endothelial cells, stromal cells, macrophages, smooth muscle cells and osteoblasts, binds to its receptor CSF1R, and exists in several isoforms- as a secreted glycoprotein, a cell-surface protein and a proteoglycan[1]. M-CSF is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts, which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes seen in patients with rheumatoid arthritis[2].

生物活性
The ED50 is <3 ng/mL as measured by murine M-NFS-60 cells, corresponding to a specific activity of >3.3 × 105 units/mg.

种属
Mouse

表达系统
E. coli

标签
Tag Free

蛋白编号
P07141 (K33-P187)

基因 ID
12977? [NCBI]

中文名
重组小鼠巨噬细胞群刺激因子
同用名
rMuM-CSF; CSF-1; MGI-IM
氨基酸序列
KEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKP

分子量
42.08

纯度
Greater than 95% as determined by reducing SDS-PAGE.

性状
无菌粉末

组分
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0.

内毒素含量
<0.2 EU/μg, determined by LAL method. 复溶方法 It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). 保存条件 & 期限 Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. 运输条件 Room temperature in continental US; may vary elsewhere. D、销售的所有产品均不得用于人类或动物之临床诊断或治疗，仅可用于工业或者科研等非医疗目的。