FGF basic 或 bFGF(基本成纤维细胞生长因子)以替代 CUG 密码子起始,标志着翻译起始的独特特征。该替代起始密码子在调节 FGF basic 的表达和功能特性中发挥着关键作用。FGF basic/bFGF 蛋白, Human (P. pastoris, N-His) 是重组的 FGF 碱性/bFGF 蛋白,由 P. pastoris 表达,带有 N-6*His 标签。FGF basic/bFGF 蛋白, Human (P. pastoris, N-His) 全长 146 个氨基酸,分子量为 17.9 kDa。
描述
FGF basic, or bFGF (fibroblast growth factor basic), initiates at an alternative CUG codon, marking a distinctive feature in translational initiation. This alternative start codon plays a pivotal role in regulating the expression and functional properties of FGF basic. FGF basic/bFGF Protein, Human (P. pastoris, N-His) is the recombinant human-derived FGF basic/bFGF protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of FGF basic/bFGF Protein, Human (P. pastoris, N-His) is 146 a.a., with molecular weight of 17.9 kDa.
研究背景
FGF basic(也称为 bFGF(碱性成纤维细胞生长因子))的起始发生在替代 CUG 密码子处。这种替代起始密码子代表了蛋白质翻译起始的独特特征,有助于形成控制 FGF basic 表达和功能特性的独特调节机制。
种属
Human
表达系统
P. pastoris
标签
N-6*His
蛋白编号
P09038-4 (P143-S288)
基因 ID
2247? [NCBI]
同用名
Fibroblast Growth Factor 2; FGF-2; Basic Fibroblast Growth Factor; bFGF; Heparin-Binding Growth Factor 2; HBGF-2; Fgf2; Fgf-2
氨基酸序列
PALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
分子量
17.9 kDa
纯度
Greater than 90% as determined by reducing SDS-PAGE.
性状
无菌粉末
组分
Lyophilized from a 0.2 μm filtered solution of Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
内毒素含量
<1 EU/μg, determined by LAL method.
复溶方法
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
保存条件 & 期限
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件
Room temperature in continental US; may vary elsewhere.
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