AB-3000B Cellgraph
Bioluminescnece imaging system for living cell and tissues
Cellgraph is an imaging system to detect low-level light emission in a single living cell by using a highly sensitive EM-CCD camera.
活细胞和组织的生物发光成像系统
Cellgraph是一种利用高灵敏度EM-CCD相机检测单个活细胞低水平光发射的成像系统。
目的和应用
实时报告基因检测
转录因子活性，时钟基因(时间药理学)。基因表达(转染,RNAi等)
细胞反应分析
药物反应(抗癌药物，DDS等)，应激反应(激素，炎症，抗氧化)，细胞毒理学
信号通路分析
信号级联(钙)、细胞凋亡等
在35mm的培养皿上获得培养细胞或组织样本的图像。
通过控制温度、CO₂气体浓度和加湿，提供CO₂培养箱等效气氛。
EM-CCD相机对微弱生物发光的高度敏感和精确检测。
分离和捕获多色微弱生物发光。
操作简单，界面友好。
用专门的软件从PC上完全控制。
完全不透光，结构紧凑。
| AB-3000B Cellgraph | |
| cooled ccD camera | ccD type:Back-illuminated EMCCD |
| Active pixel: 512 x 512 pixel | |
| pixel size:16 x 16 um | |
| ADresolution 14 / 16 bit | |
| cooling temperature | -8oC with an air-cooling system when the room temperature is 25c -90rc with a water-cooling system (optionalj |
| ccD camera water-cooling unit (optional | Constant-temperature water circulating system, heat-resistant tube for circulation |
| objective Lens | 4x(NA 0.53)(Other magnification lenses are also available as optional accessories) |
| objective lens (optional) | 2x,10 x,20 x,40x, 60 x |
| Stage | x-Y-z axis manual stage.Z-axis motorized stage |
| culture dish | 35 mm culture dish |
| co: gas injection unit (optional) | cozgas-mixer |
| co:gas humidifying unit (optional | Bubbling system for gas humidification |
| Perfusion culture chamber unit (optional) | chamber for perfusion |
| Constant temperature function | (Room temperature -5C) to 45℃,0.1℃ step |
| Lighting | white LED with dimming function Blue LED with dimming function |
| optical filter | Up to three filters can be set (525LP/560 LP/ 620 LP are standard equipment) |
| Exposure time | 30 milliseconds to 90 minutes |
| lmaging interval | Indefinite |
| Control software | os:Windows 7 / vista / xp |
| System requirement | RAM:1GB or more(512 MB or more for xP) Hard drive: 3oGB or more free disk space required lnterface: Full size Pc slot x 1, serial port x 1,uSB port x 1 |
| power (main body) | AC100-240V 106VA (aries according to the components of the whole system) |
| Dimension | 400 w x 450 (D)x 700 (H)mm |
| weight | Approx. 40 kg (Cellgraph main body only) |