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IMISX Crystallization Plate Kit
The IMISX™ Plate is designed to perform in meso in situ serial X-ray crystallography of soluble and membrane proteins [1].
The 96-well dual-sandwich IMISX™ Plate can be easily setup by hand or with an LCP dispensing robot. An SBS standard 96-well microplate footprint makes this plate compatible with standard robots and imagers.
By combining the standard LCP crystallization plate format with an advanced X-ray transparent thin-film design, the IMISX™ Plate allows for crystal growth and X-ray diffraction data collection like never before.
The thin-film sandwich design used for crystallization can be easily removed in sections and mounted onto an adaptor for use on standard goniometers for in situ X-ray diffraction data collection.
Developed in collaboration with Dr. Martin Caffrey and Dr. Meitian Wang
[1] Huang et al. (2015) In meso in situ serial X-ray crystallography of soluble and membrane proteins. Acta Cryst D 71:1238.
SKU: M-IMISX-B-01Categories: 96 Well Plates, Crystallization Plates & Accessories, In Situ Diffraction, LCP Crystallization Plates, Membrane Protein Crystallization, Membrane Protein Crystallization Plates, Starter Kits
Product Information
- Kit Content
- Product Information
- Technical Specifications
- References
IMISX™ Kit (20)-Everything needed for twenty (20) IMISX™ sandwich sets, including:
- (20) Glass LCP base plates with perimeter gaskets installed
- (20) Glass LCP top cover plates
- (40) Ultra Thin LCP Films (with protective liners) for inner sandwiches
- (20) 96-well adhesive gaskets for LCP Film sandwiches
- (1) in situ Goniometer Adaptor & holder
- (5) Goniometer Adaptor mounting gaskets
- (1) Bottle of Silanization fluid
- (6) Clean wipe applicators
- (1) Set Instructions
| Crystallize Proteins in LCP The 96-well dual-sandwich IMISX™ Plate can be easily setup by hand or with an LCP dispensing robot. An SBS standard 96-well microplate footprint makes this plate compatible with standard robots and imagers. |
 Crystals grown in an IMISX™ Plate |
| Simple and Effective By combining the standard LCP crystallization plate format with an advanced X-ray transparent thin-film design, the IMISX™ Plate allows for crystal growth and X-ray diffraction data collection like never before. |
 IMISX™ Plate Layer View |
| in situ X-ray Diffraction The thin-film sandwich design used for crystallization can be easily removed in sections and mounted onto an adaptor for use on standard goniometers for in situ X-ray diffraction data collection. |
 IMISX™ Plate Goniometer Adaptor |
Technical Specifications
For any questions about the IMISX products please call +1 607 266 877 or email [email protected].
Base plate glass (outer sandwich bottom)
| Material |
Glass |
| Thickness |
1.1 mm |
| Length |
127.7 mm |
| Width |
85.5 mm |
| Notes |
SBS compliant dimensions
works with robots and storage hotels |
Thin film (inner sandwich top and bottom)
| Material |
UV and X-ray transparent Polymer |
| Thickness |
~ 25 µm |
| Length |
112 mm |
| Width |
77 mm |
96-Well adhesive gasket
| Material |
Double-sided adhesive gasket |
| Well diameters |
5.0 mm |
| Thickness |
140 µm |
| Length |
112 mm |
| Width |
77 mm |
Top plate thin glass (outer sandwich top)
| Material |
Glass |
| Thickness |
150 µm |
| Length |
124 mm |
| Width |
88 mm |
References
[1] In meso in situ serial X-ray crystallography of soluble and membrane proteins. Huang, C.-Y., Olieric, V., Ma, P., Panepucci, E., Diederichs, K., Wang, M. & Caffrey, M. (2015). Acta Cryst. D71.
Scientific Method Commentary:
Advances in membrane protein crystallography: in situ and in meso data collection. Weyand, S. & Tate, C. G. (2015). Acta Cryst. D71, 1226-1227.
