Dasabuvir(ABT-333)
分子式：C26H27N3O5S分子量：493.57
简介： Dasabuvir(ABT-333) is a non-nucleoside inhibitor of NS5B viral RNA-dependent RNA polymerase that inhibits recombinant NS5B polymerases derived from HCV genotype 1a and 1b clinical isolates with IC50 values between 2.2 and 10.7 nM. It is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases.
物理性状及指标：
外观：……………………粉末
溶解性：…………………H2O : Insoluble ；DMSO：98 mg/mL (198.55 mM)；Ethanol：Insoluble
含量：……………………>98%
储存温度：-20℃，避光防潮密闭干燥
生物活性
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描述 |
Dasabuvir (ABT-333) 是一种非核苷抑制剂，抑制RNA依赖性的 HCV NS5B 基因编码的 RNA 聚合酶，抑制 HCV 基因型 1a 和 1b 临床分离株衍生的重组 NS5B 聚合酶，IC50 为 2.2 到 10.7 nM。 |
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IC50 & Target |
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体外 |
Dasabuvir (ABT-333) is at least 7,000-fold selective for the inhibition of HCV genotype 1 polymerases over human/mammalian polymerases. Dasabuvir (ABT-333) inhibits the polymerase enzymatic activity of genotype 1 laboratory strain enzymes (H77, BK, N, and Con1 strains), as well as enzymes produced from polymerase genes from HCV genotype 1-infected subjects, with IC50s between 2.2 and 10.7 nM. Dasabuvir (ABT-333) inhibits replication of HCV subgenomic replicons in cell culture assays, with EC50 values of 7.7 and 1.8 nM against genotype 1a (H77) and 1b (Con1), respectively. In the presence of 40% human plasma, there is a 12- to 13-fold decrease in inhibitory potency, yielding EC50s of 99 and 21 nM for HCV genotype 1a (H77) and 1b (Con1) replicons, respectively. |
储液配置及储存： 按表中溶解性配置；如溶解困难，可以通过快速搅拌，超声或温和加热（在45-60°C下水浴）。液体稳定性报道的很少，建议现配现用，如需储存，建议：-20℃1-3月；-80℃ 3-6月。
| 浓度/体积/质量 |
1 mg |
5 mg |
10 mg |
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1 mM |
2.0261 mL |
10.1303 mL |
20.2606 mL |
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5 mM |
0.4052 mL |
2.0261 mL |
4.0521 mL |
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10 mM |
0.2026 mL |
1.0130 mL |
2.0261 mL |
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50 mM |
0.0405 mL |
0.2026 mL |
0.4052 mL |
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