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JBS Crystal Dehydration and Salvage Kits
Dehydration is one of the most powerful ways for modifying (and sometimes improving) crystal diffraction properties. These kits provide a simple and cost effective approach to controlled crystal dehydration for optimizing diffraction.
JBS 水晶脱水和打捞套件
脱水是修改(有时是改进)晶体衍射特性的最有效方法之一。 这些套件提供了一种简单且经济高效的方法来控制晶体脱水以优化衍射。
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- Technical Information
Dehydration has been used as a tool for inducing structural changes in protein crystals since the earliest days of protein crystallography. Though neglected, dehydration remains a powerful tool for improving or at least modifying the diffraction properties of protein crystals.Dehydration removes excess solvent, tightens packing of protein molecules, and reduces the size of solvent channels. As a result, it sometimes improves crystal order and diffraction resolution.
By removing excess solvent, dehydration can make successful flash cooling easier, especially for crystals with large initial solvent contents.
When sufficiently dehydrated, many protein crystals undergo structural transformations, yielding alternative crystal packings that may be difficult or impossible to achieve directly during crystal growth.
Of all post-crystallization treatments, dehydration has proven to be the most effective in improving crystal diffraction properties. Of course, dehydration also often severely degrades crystal diffraction, but (amazingly!) original crystal order can usually be fully recovered just by rehydrating.
Dehydration Salts and the Crystal Dehydration and Salvage Kit have been designed for an easy, controlled and reliable way to dehydrate protein crystals and thus provide an efficient tool for altering / improving their diffraction properties.
The Dehydration Salts kit contains 12 saturated salt solutions, 1 ml each, producing relative humidities in the range of 22.5 to 97.3 %.
The Crystal Dehydration and Salvage Kit, shown above, is composed of the 12 dehydration salts, 6 Mitegen GB-B3S goniometer bases and a bottle of 20 MicroRT™ capillaries.
Dehydration Salts
Crystal Dehydration and Salvage Kit
类别:结晶优化
产品信息
细节
技术信息
从蛋白质晶体学的早期开始,脱水就被用作诱导蛋白质晶体结构变化的工具。尽管被忽视,脱水仍然是改善或至少改变蛋白质晶体衍射特性的有力工具。
脱水去除多余的溶剂,收紧蛋白质分子的堆积,并减小溶剂通道的大小。因此,它有时会提高晶体顺序和衍射分辨率。
通过去除多余的溶剂,脱水可以使成功的快速冷却更容易,特别是对于初始溶剂含量大的晶体。
当充分脱水时,许多蛋白质晶体会发生结构转变,产生替代的晶体堆积,这在晶体生长过程中可能很难或不可能直接实现。
在所有结晶后处理中,脱水已被证明是改善晶体衍射性能最有效的方法。当然,脱水通常也会严重降低晶体衍射,但是(令人惊讶的是!)通常只需通过再水化就可以完全恢复原始晶体顺序。
脱水盐和晶体脱水和回收试剂盒旨在以简单、可控和可靠的方式使蛋白质晶体脱水,从而为改变/改善其衍射特性提供有效工具。
脱水盐套件包含 12 种饱和盐溶液,每种 1 毫升,产生的相对湿度范围为 22.5 至 97.3%。
如上所示,晶体脱水和回收套件由 12 种脱水盐、6 个 Mitegen GB-B3S 测角仪底座和一瓶 20 个 MicroRT™ 毛细管组成。
脱水盐
水晶脱水和打捞工具包