日度归档：2022年10月5日

Wizard TIME(沉淀协同蛋白结晶筛选试剂盒)-MiTeGen沉淀协同蛋白结晶筛选试剂盒

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Wizard TIME (Precipitant Synergy Protein Crystallization Screen Kit)-MiTeGen沉淀协同蛋白结晶筛选试剂盒

上海金畔生物代理MiTeGen品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问MiTeGen官网或者咨询我们获取更多相关MiTeGen品牌产品信息。Wizard TIME (Precipitant Synergy Protein Crystallization Screen Kit)

Wizard TIME (Precipitant Synergy Protein Crystallization Screen Kit)

The Wizard Total Integral Membrane Protein Extraction (TIME) screen aids in the identification of the detergent reagent that successfully extracts a membrane protein from a membrane preparation. Further analysis can be carried out by ultracentrifugation, Fluorescence detection SEC-HPLC. One membrane protein target can be screened with one kit.

Wizard TIME（沉淀协同蛋白结晶筛选试剂盒）
Wizard Total Integral Membrane Protein Extraction (TIME) 筛选有助于鉴定从膜制剂中成功提取膜蛋白的去污剂试剂。 进一步的分析可以通过超速离心、荧光检测 SEC-HPLC 进行。 一种膜蛋白靶标可以用一个试剂盒进行筛选。

$445.00

SKU: M-R-1008653Category: Crystallization Screens

Product Information

  • Product Description
  • Specifications- Screen Conditions

The Wizard Total Integral Membrane Protein Extraction (TIME) screen aids in the identification of the detergent reagent that successfully extracts a membrane protein from a membrane preparation. Further analysis can be carried out by ultracentrifugation, Fluorescence detection SEC-HPLC. One membrane protein target can be screened with one kit.

note: Rigaku products are avialble through MiTeGen for customers located in the USA only.
Contact us regarding sales of these products to other regions.

Download technical sheets from these Rigaku links:Wizard TIME screen technical sheet Wizard TIME instruction sheet Wizard TIME Poster

SKU： M-R-1008653类别： 结晶筛
产品信息
产品描述
规格 – 屏幕条件
Wizard Total Integral Membrane Protein Extraction (TIME) 筛选有助于鉴定从膜制剂中成功提取膜蛋白的去污剂试剂。 进一步的分析可以通过超速离心、荧光检测 SEC-HPLC 进行。 一种膜蛋白靶标可以用一个试剂盒进行筛选。
注意：Rigaku 产品只能通过 MiTeGen 为位于美国的客户提供。
联系我们关于将这些产品销售到其他地区的事宜。

从这些理学链接下载技术表：
Wizard TIME 屏幕技术表 Wizard TIME 说明表 Wizard TIME 海报

XP Screen: The Protein Crystal Glue 96 solutions (1.7 ml each)-MiTeGen蛋白结晶筛选试剂

发表于由

XP Screen: The Protein Crystal Glue 96 solutions (1.7 ml each)-MiTeGen蛋白结晶筛选试剂

上海金畔生物代理MiTeGen品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问MiTeGen官网或者咨询我们获取更多相关MiTeGen品牌产品信息。XP Screen: The Protein Crystal Glue 96 solutions (1.7 ml each)XP Screen: The Protein Crystal Glue 96 solutions (1.7 ml each)

XP Screen: The Protein Crystal Glue 96 solutions (1.7 ml each)

The XP Screen provides 96 of the most prominent crystallization conditions upgraded with the Anderson−Evans polyoxotungstate [TeW6O24]6− (TEW) at 1 mM final concentration. This additive has proven to promote crystal contacts, improve crystal quality and further provides a valuable anomalous signal for phasing due to 6 tungsten atoms[1].
Optimized for TEW stability, the XP Screen improves crystallization and crystal diffraction quality of challenging targets.

XP Screen：The Protein Crystal Glue 96 溶液（每种 1.7 毫升）
XP Screen 提供了 96 种最突出的结晶条件，使用 1 mM 终浓度的 Anderson-Evans 多氧钨酸盐 [TeW6O24]6- (TEW) 升级。 事实证明，这种添加剂可以促进晶体接触，提高晶体质量，并由于 6 个钨原子而进一步为定相提供有价值的异常信号 [1]。
XP Screen 针对 TEW 稳定性进行了优化，可提高具有挑战性的目标的结晶和晶体衍射质量。

TEW Available Here

Full Description

SKU: M-CS-350Categories: Crystallization Screening & Optimization, Crystallization Screens, New Products

Product Information

  • Product Description
  • References
  • Specifications

The XP Screen provides 96 crystallization conditions upgraded with the Anderson−Evans polyoxotungstate [TeW6O24]6− (TEW) (available here) at 1 mM final concentration. This additive has proven to promote crystal contacts, improve crystal quality and further provides a valuable anomalous signal for phasing due to 6 tungsten atoms.

TEW was shown to:

  • covalently bind and structurally adapt to fit into protein molecules (cgAUS1, PDB code: 4Z12, 4Z13)[2]
  • act as linker in various orientations and thereby create either smaller (abPPO4, PDB code: 4OUA) or larger (lysozyme, PDB code: 4PHI) protein-protein distances[3,4]
  • induce heterogeneous crystallization of two different protein forms in one single crystal (abPPO4, PDB code: 4OUA)[4]

Our new XP Screen contains TEW in 96 of the most prominent crystallization conditions, providing an environment for TEW to improve crystallization and crystal diffraction quality especially for challenging targets.

SKU： M-CS-350 类别： 结晶筛选和优化, 结晶筛, 新产品
产品信息
产品描述
参考
规格
XP Screen 提供 96 种结晶条件，使用 1 mM 终浓度的 Anderson-Evans 多氧钨酸盐 [TeW6O24]6- (TEW)（可在此处获得）进行升级。这种添加剂已被证明可以促进晶体接触，提高晶体质量，并由于 6 个钨原子进一步提供用于定相的有价值的异常信号。

TEW 被证明：

共价结合并在结构上适应以适应蛋白质分子（cgAUS1，PDB 代码：4Z12、4Z13）[2]
作为各种方向的连接器，从而产生更小（abPPO4，PDB 代码：4OUA）或更大（溶菌酶，PDB 代码：4PHI）的蛋白质-蛋白质距离[3,4]
在一个单晶中诱导两种不同蛋白质形式的异质结晶（abPPO4，PDB 代码：4OUA）[4]
我们新的 XP Screen 在 96 种最突出的结晶条件下包含 TEW，为 TEW 提供了一个环境，以改善结晶和晶体衍射质量，尤其是对于具有挑战性的目标。

[1] Bijelic et al. (2017) Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography. Acc. Chem. Res. 50:1441.
[2] Molitor et al. (2016) In situ formation of the first proteinogenically functionalized [TeW6O24O2(Glu)]7- structure reveals unprecedented chemical and geometrical features of the Anderson-type cluster. Chem. Commun. 52:12286.
[3] Bijelic et al. (2015) Hen Egg-White Lysozyme Crystallisation: Protein Stacking and Structure Stability Enhanced by a Tellurium(VI)-Centred Polyoxotungstate. ChemBioChem 16:233.
[4] Mauracher et al. (2014) Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal. Acta Cryst. D 70:2301.

Screen Formulation: Protein Crystal Glue Formulation

MSDS: Protein Crystal Glue MSDS