EpiMark 核小体组装试剂盒
#E5350S 20 次反应
单独出售的组份
特性
概述

1 mg/ml
1 mg/ml
组蛋白 H3/a。
1 mg/ml
1 mg/ml
组蛋白 H3/m、组蛋白 H3/o。
1 mg/ml
组蛋白 H3.3A、H3F3、H3.3B。
1 mg/ml
Histone H2A combines with Histone H2B to form the H2A/H2B heterodimer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. Histones are also modified by various enzymes and can act as substrates for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the dimer may be a better substrate for specific enzymes and modifications.
Purified H2A and H2B (NEB #M2502 and NEB #M2505) were denatured, refolded and the dimer was purified by gel filtration
-20°C
20 mM Tris-HCl
2 M NaCl
1 mM DTT
1 mM EDTA
pH 8.0 @ 25°C
Histone H3.1 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. Histone H3.1, an H3 variant that has thus far only been found in mammals, is replication dependent and is associated with gene activation and gene silencing. The tetramer is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation. Because the histones are folded with their subunit partners, the tetramer may be a better substrate for specific enzymes and modifications.
Purified H3.1 and H4 (NEB #M2503 and NEB #M2504) were denatured, refolded and the Histone H3/H4 Tetramer was purified by gel filtration
Storage Temperature
-20°C
20 mM Tris-HCl
2 M NaCl
1 mM DTT
1 mM EDTA
pH 8.0 @ 25°C
Lytechinus variegatus 5SrDNA (1) of 208 bp is used for mononucleosome formation in a gel shift assay.
Plasmid (Litmus 29) containing five copies 5SrDNA is isolated from E. coli NEB 10-beta by a standard purification procedure, digested and 208 bp DNA purified
-20°C
10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C