SWISSCI结晶板 Diaplate 96 Experiment 3.2μl Capacity Dialyser

SWISSCI结晶板 Diaplate 96 Experiment 3.2μl Capacity Dialyser

Diaplate 96 Experiment 3.2μl Capacity Dialyser

Facts

  • ANSI/SLAS 1-2004 Standard
  • 96 individual membranes
  • 3.2 μl capacity

Key Features

  • Multiple application
  • Complete kit
  • High throughput use

Diaplate 96 实验 3.2μl 容量透析器
事实

ANSI/SLAS 1-2004 标准
96 个单独的膜
3.2 微升容量
主要特点

多重应用
完整的套件
高吞吐量使用

Description

About

The disposable Diaplate™ is a 96 well micro-dialysis plate ideal for the dialysis of protein from very small volumes of up to a maximum of 3.2μl.
Each of the 96 wells has a separate regenerated cellulose membrane. ANSI/ SLAS 1-2004 standards provide high-throughput use with robotic dispensing.
The SWISSCI  Diaplate™ comes as with a choice of 3, 5, 10 or 30 KDa MWCO membranes. The Diaplate™ kit includes: Diaplate with 200 Micron Pressure Adhesive Spacer, 200 Micron UVP Cover Film, UVXPO evaporation cover plate and a Sealing Paddle.

Applications

• Protein and peptide purification/concentration
• Cryo-EM Sample preparation
• Variation of crystallization conditions for crystal growth and Growth of micro-crystals for serial crystallography
• Sample preparation for biophysical assays i.e ThermoFlour®
• Removal of dyes after protein labeling
• Electro-elution of proteins
• Protein and DNA desalting/buffer exchange
• Protein in vitro translation
• Glycoprotein modification and engineering
• Enzyme activity/binding assays/detergent removal

Product Specifications

• Simple handling, ready to use, easy sample recovery
• Complies with ANSI/SLAS standards for automation
• Pipetting can be performed manually or using a robot
• Ideal for the desalting of up to 3.2μl protein solution
• No cross-contamination or leakage between wells guaranteed
• 96 individual regenerated cellulose membranes; sulfur and heavy metal-free
• A choice of different MW cut-off membranes
• Protein crystallization applications allow for unique matrix changing
• Low protein binding and high protein retention
• Dialysis complete in 2-4 hours
• Gentle and efficient desalting without disturbing valuable protein complexes

描述

关于

一次性 Diaplate™ 是一款 96 孔微量透析板,非常适合从极小体积到最大 3.2μl 的蛋白质透析。
96 个孔中的每一个都有一个单独的再生纤维素膜。 ANSI/ SLAS 1-2004 标准为机器人点胶提供了高通量使用。
SWISSCI Diaplate™ 可选择 3、5、10 或 30 KDa MWCO 膜。 Diaplate™ 套件包括:带 200 微米压力粘合剂垫片、200 微米 UVP 覆盖膜、UVXPO 蒸发盖板和密封垫的 Diaplate。

应用

• 蛋白质和肽纯化/浓缩
• 冷冻电镜样品制备
• 晶体生长的结晶条件变化和连续晶体学的微晶体生长
• 用于生物物理分析的样品制备,例如 ThermoFlour®
• 蛋白质标记后去除染料
• 蛋白质的电洗脱
• 蛋白质和 DNA 脱盐/缓冲液交换
• 蛋白质体外翻译
• 糖蛋白修饰和工程
• 酶活性/结合测定/去污剂

产品规格

• 操作简单,即用型,样品回收方便
• 符合 ANSI/SLAS 自动化标准
• 可以手动或使用机器人进行移液
• 最适合脱盐高达 3.2μl 的蛋白质溶液
• 保证井之间没有交叉污染或泄漏
• 96 个单独的再生纤维素膜;不含硫和重金属
• 可选择不同的 MW 截止膜
• 蛋白质结晶应用允许独特的基质变化
• 低蛋白结合和高蛋白保留
• 透析在 2-4 小时内完成
• 温和高效的脱盐,不会干扰有价值的蛋白质复合物

Order Information

Item: Diaplate
Code: W82003; W82005; W82010; W82030.
Details: Pack of 3

Diaplate Crystallisation Method

Related products

SWISSCI 96孔结晶板 MRC Microbatch Under Oil Plate

SWISSCI 96孔结晶板 MRC Microbatch Under Oil Plate

MRC Microbatch Under Oil Plate

Facts

  • ANSI/SLAS 1-2004 Standard
  • 96 wells
  • 20 micro-liter volume

Key Features

  • Optically perfect wells
  • Micro-numbering
  • Optimized for micro-batch crystallization
 MRC Microbatch 下油盘
事实

ANSI/SLAS 1-2004 标准
96口孔
20微升体积
主要特点

光学完美的孔
微编号
针对微批量结晶进行了优化

Description

About

The SWISSCI MRC Under Oil 96 Well Crystallisation plate uses oil as a seal and active diffusion mechanism for the purpose of running macromolecular crystallization under oil.
The plate offers unique properties that make it ideal for both nanolitre crystallisation screening and microlitre optimisation alike. The plate was developed at the MRC Laboratory of Molecular Biology (Cambridge, UK) in collaboration with Dr. Jan Löwe and colleagues. It is a result of many years of experience in successful robotic high-throughput crystallization.

Product Specifications

Easy Crystal Retrieval
Raised wide wells make crystal mounting especially easy

Easy Viewing
The micro-numbering is visible by microscope.
The wells are a wide conical shape and have a lens effect for perfect illumination.
UV transmissible polymer

Recommended Volumes
Typical volumes validated for these plates are 20 micro-liters of oil with a shot through sample delivery of 100 to 200 nano-liters.
The 20 micro-liter volume of the individual wells gives the user a wide range of macromolecular crystallization possibilities.

ANSI/SLAS 1-2004 Standard
The plates are designed to the 96 – well ANSI/SLAS 1-2004 standards for all common holders and external numbering (A – H, 1 – 12) with corner location that make the plate easy to use in a robotic sampler.
The plate can also be centrifuged for better results.
The unique MRC Under Oil 96 well Crystallisation plate offers a new approach to micro-batch crystallography.

描述
关于

SWISSCI MRC Under Oil 96 Well Crystallisation 板使用油作为密封和主动扩散机制,目的是在油下进行大分子结晶。
该板具有独特的特性,使其成为纳升结晶筛选和微升优化等的理想选择。该板是在 MRC 分子生物学实验室(英国剑桥)与 Jan Löwe 博士及其同事合作开发的。这是多年成功的机器人高通量结晶经验的结果。

产品规格
简单的水晶检索
凸起的宽孔使晶体安装特别容易

轻松查看
显微镜下可以看到微编号。
孔为宽锥形,具有透镜效应,可实现完美照明。
可透过紫外线的聚合物

推荐卷
经验证的这些板的典型体积为 20 微升油,样品输送量为 100 至 200 纳升。
单个孔的 20 微升体积为用户提供了广泛的大分子结晶可能性。

ANSI/SLAS 1-2004 标准
这些板的设计符合 96 孔 ANSI/SLAS 1-2004 标准,适用于所有常见的支架和外部编号(A – H、1 – 12),带有角落位置,使板易于在机器人采样器中使用。
该板也可以离心以获得更好的结果。
独特的 MRC Under Oil 96 孔结晶板为微批量结晶学提供了一种新方法。

Order Information

Item: MRC Under-oil plate
Code: UO96T-UVP
Details: Box of 100